APOBEC3B is preferentially expressed at the G2/M phase of cell cycle

APOBEC3B (A3B) is a cytosine deaminase that converts to uracil in single-stranded DNA. Cytosine-to-thymine and cytosine-to-guanine base substitution mutations trinucleotide motifs (APOBEC mutational signatures) were found various cancers including lymphoid hematological malignancies such as multiple myeloma A3B has been shown be an enzymatic source of those cancers. Although the importance being increasingly recognized, it unclear how expression regulated cancer cells well normal cells. To answer these fundamental questions, we analyzed 1276 primary using single-cell RNA-sequencing (scRNA-seq) was preferentially expressed at G2/M phase, sharp contrast patterns other APOBEC3 genes. Consistently, demonstrated protein phase by cell sorting. We also blood expressing enriched G2/M-phase analyzing scRNA-seq data from 86,493 bone marrow mononuclear Furthermore, revealed mainly plasma cells, CD10+ B erythroid but not granulocyte-macrophage progenitors. profiling may contribute understanding defense mechanism against viruses, partially explain bias APOBEC signatures myeloid malignancies. This study identified cellular which highly expressed, help reveal mechanisms behind carcinogenesis heterogeneity, biological functions